|
KMID : 0350519940470020823
|
|
Journal of Catholic Medical College
1994 Volume.47 No. 2 p.823 ~ p.835
|
|
|
Effect of Percutaneous Autograft With Bone Marrow on Segmental Diaphyseal Defect in Rabbits
|
|
|
|
|
Abstract
|
|
|
The purpose of this study was to investigate the effect of autograft with bone marrow on the repair or bone formation of the femoral diaphyseal defects in rabbits, by the serial radiogram, bone densitometry, morphology and histology. On the left
femoral
shaft of thirty-six adult rabbits weighing from 2,500gm to 3,000gm, 2cm of segmental bone resection was performed and then the ostectomized femur was internally fixated with plate to keep the length of the bony defect. Animals were allocated into
three
groups ; Group I was percutaneous saline injection on the bony defect site at the day of operation(control group), Group Ⅱwas immediate autograft with bone marrow on the bony defect site at the day of operation(experimental group) and group
Ⅲwas
autograft with bone marrow on the bony defect site at one week after operation(experimental group). Serial radiograms were taken at 1, 2, 3 and 4 weeks after operation for the assessment of bridging callus formation. At 2 weeks after operation,
four
rabbits of each group were sacrificed and the changes of histologic findings were observed. At 4 weeks after operation, bone densitometry, histology of the original defect site as well as the ratio of diameter of the callus and that of unoperated
femur
were assessed.
@ES The results were as follows:
@EN 1. Radiologically significant amount of callus was formed at 2 weeks after operation in group Ⅱand at 3 weeks in group Ⅲ. At 4 weeks after operation, significant amount of callus was formed in group Ⅱand group Ⅲcompared to that of group
I.
But
there was no significant difference between group Ⅱand group Ⅲ.
2. The bone mineral density which was measured at 4 weeks after operation was 0.022±0.004g/cm* in group Ⅱand 0.042±0.003g/cm* in group Ⅲ. The bone mineral density was significantly higher in group Ⅱand group Ⅲthan that of group I
(p<0.005),
and
there was no significant difference between group Ⅱand group Ⅲ.
3. The ratio of diameter of unoperated femur and the callus of operated femur of each group at post-operation 4 weeks were 90.85±13.9 in group I, 94.46±10.5 in group Ⅱand 92.53±7.7 in group Ⅲand there was no significant difference among
three
groups(p>0.05).
4. At 2 weeks after operation, in group I, Immature fibrous tissue, in group Ⅱ, several trabecular bony islands and in group Ⅲ, immature catilage, small bony island and fibrous tissue were observed histologically on the bony defect site. At 4
weeks
after operation, the fibro-osseous bridging was found in group I on the bony defect area. The trabecular bony bridging was found in group Ⅱand group Ⅲbut the osseous callus was more matured in group Ⅱ.
From this results, it was demonstrated that autograft with bone marrow hastens to form direct osseus callus formation at the bony defect site. The efficacy on the autograft with bone marrow does not seemed to be related with the inflammatory
phase
of
the fracture healing process, and we think earlier autograft with bone marrow maybe efficacious to treat delayed and nonunited fracture as a clinical setting with easy application. ±±
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|